Expression vectors
Go back to the Protein Expression and Purification Core Facility site| Name | Map | Antibiotic resistance | Promoter | Fusion tags | Protease cleavage site | Source | Remarks |
|---|---|---|---|---|---|---|---|
| pHIL-D2 | Sequence | Amp | AOX1 | None | None | Invitrogen | Pichia pastoris HIS4 vector for methanol-inducible intracellular expression of a protein |
| pHIL-S1 | Sequence | Amp | AOX1 | None | None | Invitrogen | Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein (PHO1 signal sequence) |
| pIB1 | Sequence | Amp | None | None | None | B. Glick | |
| pIB2 | Sequence | Amp | GAP | None | None | B. Glick | Pichia pastoris expression vector |
| pIB3 | Sequence | Amp | YPT1 | None | None | B. Glick | Pichia pastoris expression vector |
| pIB4 | Sequence | Amp | AOX1 | None | None | B. Glick | Pichia pastoris expression vector |
| pPIC3.5 | Sequence | Amp | AOX1 | None | None | Invitrogen | Pichia pastoris multi-copy integrating vector for methanol-inducible intracellular expression of a protein |
| pPIC9 | Sequence | Amp | AOX1 | Nt s-factor secretion signal (presequence-pro region) | None | Invitrogen | Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein |
| pPIC9K | Sequence | Amp, Kan | AOX1 | Nt s-factor secretion signal (presequence-pro region) | None | Invitrogen | Pichia pastoris HIS4 vector for methanol-inducible expression of a secreted protein |