Expression vectors
Go back to the Protein Expression and Purification Core Facility siteName | Map | Antibiotic resistance | Promoter | Fusion tags | Protease cleavage site | Source | Remarks |
---|---|---|---|---|---|---|---|
pCoofy1 | Sequence | Kan | T7/lac | Nt His6 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy2 | Sequence | Kan | T7/lac | Nt Trx-His6 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy4 | Sequence | Kan | T7/lac | Nt His6-MBP | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy5 | Sequence | Kan | T7/lac | Nt His6-thrombin-hSumo1 | thrombin, hSenP2 | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy6 | Sequence | Kan | T7/lac | Nt His6-thrombin-hSumo3 | thrombin, hSenP3 | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy7 | Sequence | Kan | T7/lac | Nt S-tag | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy8 | Sequence | Kan | T7/lac | Nt HaloTag | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy9 | Sequence | Amp | T7/lac | Nt StrepII, Ct His6 | 3C, thrombin | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy12 | Sequence | Kan | T7/lac | Nt TwinStrepII | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy16 | Sequence | Kan | T7/lac | Nt His10-NusA | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy18 | Sequence | Kan | T7/lac | Nt His10 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy21 | Sequence | Kan | T7/lac | Nt His10-TwinStrepII | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy24 | Sequence | Kan | T7/lac | Nt CBP, Ct 10His | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy26 | Sequence | Kan | T7/lac | Nt NusA, Ct His10 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy31 | Sequence | Kan | T7/lac | Nt TwinStrepII, Ct His6 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy33 | Sequence | Kan | T7/lac | Nt His10, Ct TwinStrepII | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy34 | Sequence | Kan | T7/lac | Nt S-tag-TwinStrepII | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy35 | Sequence | Kan | T7/lac | Nt MBP | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy36 | Sequence | Kan | T7/lac | Nt S-tag-TwinStrepII, Ct His10 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy37 | Sequence | Kan | T7/lac | Nt MBP, Ct His6 | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy49 | Sequence | Kan | T7/lac | Nt His6-MBP, Ct His10 or TwinStrepII or S-tag or CBP | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy52 | Sequence | Kan | T7/lac | Nt His6-AVI-AVI-AVI | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy61 | Sequence | Kan | T7/lac | Nt His6-eGFP | 3C | Sabine Suppmann, MPIB | Bacterial LIC-adapted expression vector |
pCoofy27 | Sequence | Amp, Gent | polyhedrin | Nt His7 | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy28 | Sequence | Amp, Gent | polyhedrin | Nt His6-GST | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy29 | Sequence | Amp, Gent | polyhedrin | Nt His6-MBP | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy41 | Sequence | Amp, Gent | polyhedrin | Ct His10 or TwinStrepII or S-tag or CBP | no | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy51 | Sequence | Amp, Gent | polyhedrin | Nt TwinStrepII | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy59 | Sequence | Amp, Gent | polyhedrin | Nt TrxA-His6 | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy60 | Sequence | Amp, Gent | polyhedrin | Nt His6-eGFP | 3C | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy63 | Sequence | Amp, Gent | polyhedrin | Nt His6-SumoStar, Ct His10 or TwinStrepII or EPEA | SumoStar protease | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy64 | Sequence | Amp, Gent | polyhedrin | Nt GP67ss-His6-SumoStar, Ct His10 or TwinStrepII or EPEA | SumoStar protease | Sabine Suppmann, MPIB | Insect cell LIC-adapted expression vector |
pCoofy47 | Sequence | Amp | CMV | Nt VEGFss, Ct His10 or TwinStrepII or S-tag or CBP | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pTT5 backbone (Durocher et al., 2002, NAR) |
pCoofy50 | Sequence | Amp | EF1alpha | Nt HA-His8-FLAG, Ct His10 | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pEF-HA backbone |
pCoofy55 | Sequence | Amp | CMV | Nt His10-SumoStar, Ct His10 or TwinStrepII or S-tag | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pTT5 backbone (Durocher et al., 2002, NAR) |
pCoofy56 | Sequence | Amp | EF1alpha | Nt TwinStrepII, Ct His10 | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pEF-HA backbone |
pCoofy57 | Sequence | Amp | CMV | Nt VEGFss-His8, Ct His10 or TwinStrepII or S-tag or CBP | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pTT5 backbone (Durocher et al., 2002, NAR) |
pCoofy58 | Sequence | Amp | CMV | Nt VEGFss-twinStrepII, Ct His10 or TwinStrepII or S-tag or CBP | 3C | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pTT5 backbone (Durocher et al., 2002, NAR) |
pCoofy62 | Sequence | Amp | CMV | Nt VEGFss-His6-SumoStar, Ct His10 or TwinStrepII or S-tag or CBP | SumoStar protease | Sabine Suppmann, MPIB | Mammalian LIC-adapted expression vector; pTT5 backbone (Durocher et al., 2002, NAR) |