Protein Expression and Purification Core Facility

Expression vectors

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Expression vectors for mammalian cells

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Name Map Antibiotic resistance Promoter Fusion tags Protease cleavage site Source Remarks
pACEMam1 Sequence Gent CMV None None Geneva Biotech MultiMam acceptor vector
pACEMam2 Sequence Gent CAG None None Geneva Biotech MultiMam acceptor vector
pMDC Sequence Cam CMV None None Geneva Biotech MultiMam donor vector
pMDK Sequence Kan CAG None None Geneva Biotech MultiMam donor vector
pMDS Sequence Spect CAG None None Geneva Biotech MultiMam donor vector
pACEMam_intron_Kozak Sequence Gent CMV None None Martin Wuertz
pACEMam_intron_Kozak_eGFP Sequence Gent CMV eGFP None Martin Wuertz
pHTBV1.1-CT10H-SIII-LIC Vector sheet Amp, Gent CMV Ct His10-twinStrepII TEV Nicola Burgess-Brown, SGC LIC-adapted BacMam vector based on the pHTBV1.1 from Frederick Boyce, Harvard
pHTBV1.1-CTGFP-SIII-10H-LIC Sequence Amp, Gent CMV Ct GFP-twinStrepII-His10 TEV Nicola Burgess-Brown, SGC LIC-adapted BacMam vector based on the pHTBV1.1 from Frederick Boyce, Harvard
pHTBV1.1-NSIII-10H-GFPT-LIC Sequence Amp, Gent CMV Nt twinStrepII-His10 TEV Nicola Burgess-Brown, SGC LIC-adapted BacMam vector based on the pHTBV1.1 from Frederick Boyce, Harvard
pHTBV1.1-NSIII-10H-LIC Vector sheet Amp, Gent CMV Nt twinStrepII-His10-GFP TEV Nicola Burgess-Brown, SGC LIC-adapted BacMam vector based on the pHTBV1.1 from Frederick Boyce, Harvard
pHBTV1.1-SecNH-Bio-LIC Sequence Amp, Gent CMV Nt RPTPmu signal sequence-His10, Ct AVI-tag TEV Nicola Burgess-Brown, SGC LIC-adapted BacMam vector based on the pHTBV1.1 from Frederick Boyce, Harvard
pXLG Sequence Amp CMV eGFP None David Hacker, EPFL