Expression vectors
Go back to the Protein Expression and Purification Core Facility siteVectors that are commercially available can only be shared with internal EMBL users, but vectors generated at EMBL are freely available to the entire academic research community via a Material Transfer Agreement. Vectors that can be shared with external users are indicated in yellow in the vector tables.
| Name | Map | Antibiotic resistance | Promoter | Fusion tags | Protease cleavage site | Source | Remarks |
|---|---|---|---|---|---|---|---|
| pACEBac1 | Sequence | Gent | polyhedrin | None | None | Imre Berger | MultiBac acceptor vector |
| pACEBAC1-EGT | Sequence | Gent | polyhedrin | None | None | PEPCF | EGT signal sequence |
| pACEBAC1-GP67long | Sequence | Gent | polyhedrin | None | None | PEPCF | GP64/GP67 signal sequence (long) |
| pACEBAC1-GP67short | Sequence | Gent | polyhedrin | None | None | PEPCF | GP64/GP67 signal sequence (short) |
| pACEBAC1-HBM | Sequence | Gent | polyhedrin | None | None | PEPCF | HBM signal sequence |
| pACEBac2 | Sequence | Gent | p10 | None | None | Imre Berger | MultiBac acceptor vector |
| pBIG1a | Sequence | Gent, Amp, Spec | None | None | None | Addgene | biGBac vector |
| pBIG1b | Sequence | Gent, Amp, Spec | None | None | None | Addgene | biGBac vector |
| pBIG1c | Sequence | Gent, Amp, Spec | None | None | None | Addgene | biGBac vector |
| pBIG1d | Sequence | Gent, Amp, Spec | None | None | None | Addgene | biGBac vector |
| pBIG1e | Sequence | Gent, Amp, Spec | None | None | None | Addgene | biGBac vector |
| pBIG2a | Sequence | Gent, Amp, Cam | None | None | None | Addgene | biGBac vector |
| pBIG2ab | Sequence | Gent, Amp, Cam | None | None | None | Addgene | biGBac vector |
| pBIG2abc | Sequence | Gent, Amp, Cam | None | None | None | Addgene | biGBac vector |
| pBIG2abcd | Sequence | Gent, Amp, Cam | None | None | None | Addgene | biGBac vector |
| pFastBac-Dual | Sequence | Gent, Amp | polyhedrin, p10 | None | None | Life Technologies | Co-expression vector |
| pFastBac ET | Sequence | Gent, Amp | polyhedrin | None | None | A. Geerlof | |
| pFastBac HTa | Sequence | Gent, Amp | polyhedrin | Nt His6 | TEV | Life Technologies | |
| pFastBac HTb | Sequence | Gent, Amp | polyhedrin | Nt His6 | TEV | Life Technologies | |
| pFastBac HTc | Sequence | Gent, Amp | polyhedrin | Nt His6 | TEV | Life Technologies | |
| pFastBac-M30a | Sequence | Gent, Amp | polyhedrin | Nt GST | 3C | EMBL | |
| pFastBac-M30b | Sequence | Gent, Amp | polyhedrin | Nt GST | 3C | EMBL | |
| pFastBac-M30c | Sequence | Gent, Amp | polyhedrin | Nt GST | 3C | EMBL | |
| pFastBac1 | Sequence | Gent, Amp | polyhedrin | None | None | Life Technologies | |
| pIDC | Sequence | Cam | polyhedrin | None | None | Imre Berger | MultiBac donor vector |
| pIDK | Sequence | Spect | p10 | None | None | Imre Berger | MultiBac donor vector |
| pIDS | Sequence | Kan | p10 | None | None | Imre Berger | MultiBac donor vector |
| pLIB | Sequence | Gent, Amp | polyhedrin | None | None | Addgene | biGBac vector |
| pVL1392 | Sequence | Amp | polyhedrin | None | None | Invitrogen | Baculovirus transfer vector (recombination-based) |
| pVL1393 | Sequence | Amp | polyhedrin | None | None | Invitrogen | Baculovirus transfer vector (recombination-based) |
| pVL1393-M10 | Sequence | Amp | polyhedrin | Nt His6 | None | H v/d Zandt | Baculovirus transfer vector (recombination-based) |
| pVL1393-M11 | Sequence | Amp | polyhedrin | Ct His6 | None | H v/d Zandt | Baculovirus transfer vector (recombination-based) |